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Recently, the first point-of-care molecular diagnostic test was cleared by the U.S. Food and Drug Administration for detection of the flu. At the same time, concerns about the performance of commonly used rapid antigen tests have surfaced. The landscape of influenza diagnostics is rapidly evolving and clinical laboratories are certain to face pressure regarding new testing modalities.
A Q&A feature in the November 2018 issue of Clinical Chemistry asked five experts with different roles in this field to discuss recent advances and ongoing challenges in influenza diagnostics.
]]>Recently, the first point-of-care molecular diagnostic test was cleared by the U.S. Food and Drug Administration for detection of the flu. At the same time, concerns about the performance of commonly used rapid antigen tests have surfaced. The landscape of influenza diagnostics is rapidly evolving and clinical laboratories are certain to face pressure regarding new testing modalities.
A Q&A feature in the November 2018 issue of Clinical Chemistry asked five experts with different roles in this field to discuss recent advances and ongoing challenges in influenza diagnostics.
]]>An original research article appearing in the July 2018 issue of Clinical Chemistry describes these achievements while establishing the analytical validity of expanded carrier screening and quantifying how many pregnancies could be impacted by this approach in the general U.S. population.
]]>An original research article appearing in the July 2018 issue of Clinical Chemistry describes these achievements while establishing the analytical validity of expanded carrier screening and quantifying how many pregnancies could be impacted by this approach in the general U.S. population.
]]>Despite these advantages, use of saliva for understanding more about extracellular RNA and its role in human biology and disease through RNA sequencing has some challenges. The high bacterial content and low abundance of extracellular RNA in saliva mean that optimization of RNA library construction and isolation protocols are critical to the success of these RNA sequencing experiments.
An original research article in the July 2018 issue of Clinical Chemistry compares different RNA isolation methods and library construction kits for long and small RNA sequencing of salivary extracellular RNA. The authors described which protocols provide the best RNA yield and detection by next generation sequencing.
]]>Despite these advantages, use of saliva for understanding more about extracellular RNA and its role in human biology and disease through RNA sequencing has some challenges. The high bacterial content and low abundance of extracellular RNA in saliva mean that optimization of RNA library construction and isolation protocols are critical to the success of these RNA sequencing experiments.
An original research article in the July 2018 issue of Clinical Chemistry compares different RNA isolation methods and library construction kits for long and small RNA sequencing of salivary extracellular RNA. The authors described which protocols provide the best RNA yield and detection by next generation sequencing.
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Beyond the minimal invasiveness, performing liquid biopsy may overcome some other weaknesses of tissue biopsy that are related to a single site's inability to capture intratumor heterogeneity or be representative of all the changes within the tumor. All three approaches to detect a tumor's genetic material have been applied for therapy stratification and monitoring in breast cancer. However, knowledge about the differences between these methods in the same patient cohort is limited.
An original article in the July 2018 issue of Clinical Chemistry compared messenger RNA profiles of circulating tumor cells and extracellular vesicles in patients with metastatic breast cancer to estimate the utility of each in therapy management.
Beyond the minimal invasiveness, performing liquid biopsy may overcome some other weaknesses of tissue biopsy that are related to a single site's inability to capture intratumor heterogeneity or be representative of all the changes within the tumor. All three approaches to detect a tumor's genetic material have been applied for therapy stratification and monitoring in breast cancer. However, knowledge about the differences between these methods in the same patient cohort is limited.
An original article in the July 2018 issue of Clinical Chemistry compared messenger RNA profiles of circulating tumor cells and extracellular vesicles in patients with metastatic breast cancer to estimate the utility of each in therapy management.
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As more recent studies have identified additional cardiac troponin I proteolytic fragments, it is important to understand their composition and prevalence in the blood of patients with acute myocardial infarction. Furthermore, there is a need to evaluate whether monoclonal antibodies specific to the regions beyond the central section of the molecule can be used to develop cardiac troponin I assays with improved performance over those currently available. An original article appearing in the July 2018 issue of Clinical Chemistry describes a study investigating these concepts.
]]>As more recent studies have identified additional cardiac troponin I proteolytic fragments, it is important to understand their composition and prevalence in the blood of patients with acute myocardial infarction. Furthermore, there is a need to evaluate whether monoclonal antibodies specific to the regions beyond the central section of the molecule can be used to develop cardiac troponin I assays with improved performance over those currently available. An original article appearing in the July 2018 issue of Clinical Chemistry describes a study investigating these concepts.
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In the April 2018 issue of Clinical Chemistry, a paper demonstrated the feasibility of generating an accurate whole genome sequence of a fetus from either the cellular or cell-free DNA of an amniotic sample.
In the April 2018 issue of Clinical Chemistry, a paper demonstrated the feasibility of generating an accurate whole genome sequence of a fetus from either the cellular or cell-free DNA of an amniotic sample.
]]>The January 2017 Clinical Chemistry special issue on obesity includes a review article that summarizes the current and future roles of nutritional metabolomics.
]]>The January 2017 Clinical Chemistry special issue on obesity includes a review article that summarizes the current and future roles of nutritional metabolomics.
]]>Though promising evidence is emerging, there is a need for further mechanistic studies to assess the true potential of metabolic surgery to treat the myriad other disorders of metabolism and to better understand their consequences in terms of cardiovascular disease and cancer risk reduction. Additionally, there may be unintended consequences of bariatric surgery that are related to long term adverse skeletal effects and nutritional deficiencies.
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Though promising evidence is emerging, there is a need for further mechanistic studies to assess the true potential of metabolic surgery to treat the myriad other disorders of metabolism and to better understand their consequences in terms of cardiovascular disease and cancer risk reduction. Additionally, there may be unintended consequences of bariatric surgery that are related to long term adverse skeletal effects and nutritional deficiencies.
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A Clinical Case Study in the December 2017 issue of Clinical Chemistry discusses a series of adult patients who presented with similar symptoms and were discovered to have extremely elevated blood lead concentrations.
A Clinical Case Study in the December 2017 issue of Clinical Chemistry discusses a series of adult patients who presented with similar symptoms and were discovered to have extremely elevated blood lead concentrations.
]]>Cell lines derived from circulating tumor cells can be created to overcome this problem. This has been done in colon cancer, for example, and enables researchers to examine molecular and functional differences between the circulating tumor cells and those from the primary tumor. In this way, researchers may further our ability to identify and characterize cells that initiate metastasis and to ultimately develop new therapies to stop them.
An original report in the January 2017 issue of Clinical Chemistry describes the differential gene expression between colon cancer cell lines derived from circulating tumor cells and the primary tumor. Dr. Catherine Alix-Panabières is the primary author of this article and joins us for this podcast.
]]>Cell lines derived from circulating tumor cells can be created to overcome this problem. This has been done in colon cancer, for example, and enables researchers to examine molecular and functional differences between the circulating tumor cells and those from the primary tumor. In this way, researchers may further our ability to identify and characterize cells that initiate metastasis and to ultimately develop new therapies to stop them.
An original report in the January 2017 issue of Clinical Chemistry describes the differential gene expression between colon cancer cell lines derived from circulating tumor cells and the primary tumor. Dr. Catherine Alix-Panabières is the primary author of this article and joins us for this podcast.
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