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Global Epigenetics Market 2019-2025 to Grow at an Impressive Pace

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(EMAILWIRE.COM, October 05, 2019 ) OG Analysis, a global market research firm, has announced the release of their “Global Epigenetics Market Size, Outlook and Growth Opportunities to 2025: By Product (Kits, Reagents, Enzymes, Instruments and Consumables, and Bioinformatics Tools), By Application...
          

Come diventare Bioinformatico

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Ciao a tutti. Sono laureato in scienze agrarie e mi sto specializzando in biotecnologie vegetali. Durante la magistrale ho fatto un esame di Bioinformatica che mi è piaciuto molto al punto da decidere di fare il tirocinio in tale ambito. Infatti ho deciso di voler intraprendere questa carriera ma......

Ci sono 1 risposta/e, l'ultimo messaggio è stato inserito il 07 ottobre 2019 alle 21:51:11 da domi84
Forum: Laboratorio > Bioinformatica e Biostatistica > Come diventare Bioinformatico
          

GFP tag

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Per quale motivo alcuni dei geni taggati con GFP non emettono fluorescenza?
Ed a quali altri errori si può andare incontro utilizzando questa tecnica?
Grazie

Ci sono 1 risposta/e, l'ultimo messaggio è stato inserito il 07 ottobre 2019 alle 11:15:53 da omarl
Forum: Laboratorio > Bioinformatica e Biostatistica > GFP tag
          

Novel ribozymes: discovery, catalytic mechanisms, and the quest to understand biological function

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Abstract
Small endonucleolytic ribozymes promote the self-cleavage of their own phosphodiester backbone at a specific linkage. The structures of and the reactions catalysed by members of individual families have been studied in great detail in the past decades. In recent years, bioinformatics studies have uncovered a considerable number of new examples of known catalytic RNA motifs. Importantly, entirely novel ribozyme classes were also discovered, for most of which both structural and biochemical information became rapidly available. However, for the majority of the new ribozymes, which are found in the genomes of a variety of species, a biological function remains elusive. Here, we concentrate on the different approaches to find catalytic RNA motifs in sequence databases. We summarize the emerging principles of RNA catalysis as observed for small endonucleolytic ribozymes. Finally, we address the biological functions of those ribozymes, where relevant information is available and common themes on their cellular activities are emerging. We conclude by speculating on the possibility that the identification and characterization of proteins that we hypothesize to be endogenously associated with catalytic RNA might help in answering the ever-present question of the biological function of the growing number of genomically encoded, small endonucleolytic ribozymes.

          

Come diventare Bioinformatico

 Cache   
Ciao a tutti. Sono laureato in scienze agrarie e mi sto specializzando in biotecnologie vegetali. Durante la magistrale ho fatto un esame di Bioinformatica che mi è piaciuto molto al punto da decidere di fare il tirocinio in tale ambito. Infatti ho deciso di voler intraprendere questa carriera ma......

Ci sono 1 risposta/e, l'ultimo messaggio è stato inserito il 07 ottobre 2019 alle 21:51:11 da domi84
Forum: Laboratorio > Bioinformatica e Biostatistica > Come diventare Bioinformatico
          

GFP tag

 Cache   
Per quale motivo alcuni dei geni taggati con GFP non emettono fluorescenza?
Ed a quali altri errori si può andare incontro utilizzando questa tecnica?
Grazie

Ci sono 1 risposta/e, l'ultimo messaggio è stato inserito il 07 ottobre 2019 alle 11:15:53 da omarl
Forum: Laboratorio > Bioinformatica e Biostatistica > GFP tag
          

Long and Repeat-Rich Intronic Sequences Favor Circular RNA Formation under Conditions of Reduced Spliceosome Activity.

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Long and Repeat-Rich Intronic Sequences Favor Circular RNA Formation under Conditions of Reduced Spliceosome Activity.

iScience. 2019 Sep 06;20:237-247

Authors: Wang M, Hou J, Müller-McNicoll M, Chen W, Schuman EM

Abstract
Circular RNAs (circRNAs), an important class of regulatory RNAs, have been shown to be the most prevalent in the brain compared with other tissues. However the processes governing their biogenesis in neurons are still elusive. Moreover, little is known about whether and how different biogenesis factors work in synchrony to generate neuronal circRNAs. To address this question, we pharmacologically inhibited the spliceosome and profiled rat neuronal circRNAs using RNA sequencing. We identified over 100 circRNAs that were up-regulated and a few circRNAs that were down-regulated upon spliceosome inhibition. Bioinformatic analysis revealed that up-regulated circRNAs possess significantly longer flanking introns compared with the un-changed circRNA population. Moreover, the flanking introns of up-regulated circRNAs harbor a higher number of distinct repeat sequences and more reverse complementary motifs compared with the unchanged circRNAs. Taken together, our data demonstrate that the biogenesis of circRNAs containing distinct intronic features becomes favored under conditions of limited spliceosome activity.

PMID: 31590076 [PubMed - as supplied by publisher]




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